Part:BBa_K2959002:Design

Truncated Saccharomyces cerevisiae FAD-linked sulfhydryl oxidase Erv1p

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 58
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence consists in a truncated version of Erv1p that lacks the first 72 amino acids of the full sequence. It has been proved that this version shows similar or even improved sulfhydryl oxidase activity¹.

Source

The complete amino acid sequence was reported by Lisowsky (1996). The truncated Erv1p amino acid sequence was retrieved from Lee et al. (2000).

References

1. Lee, J. E., Hofhaus, G., & Lisowsky, T. (2000). Erv1p from Saccharomyces cerevisiae is a FAD‐linked sulfhydryl oxidase. FEBS letters, 477(1-2), 62-66. doi: 10.1016/s0014-5793(00)01767-1

2. Lisowsky, T. (1996). Removal of an intron with unique 3′ branch site creates an amino‐terminal protein sequence directing the scERV1 gene product to mitochondria. Yeast, 12(15), 1501-1510. doi: 10.1002/(SICI)1097-0061(199612)12:15%3C1501::AID-YEA40%3E3.0.CO;2-H